Effect of antitumor alkylating agents on semiconservative and unscheduled DNA syntheses in rat ascites hepatoma cells.
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چکیده
منابع مشابه
Induction of unscheduled DNA synthesis in human bone marrow cells by bifunctional alkylating agents.
A technique has been developed for handling human bone marrow cells intended for the examination of DNA repair synthesis. DNA-repair synthesis, induced by melphalan and nitrogen mustard, was measured as the incorporation of 3H-thymidine, registered by autoradiography as unscheduled DNA synthesis (UDS). Comparison of various cell populations disclosed considerable differences in their UDS level,...
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protein tyrosine phosphatases (ptpases) regulate tyrosine phosphorylation of target proteins involved in several aspects of cellular functions. enzyme activities of the ptpases in cytosolic and particulate fractions of rat ascites hepatoma cell lines were determined and compared with those of normal rat liver. our present data revealed that although there was no neoplatic-specific alteration of...
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Treatment of Ehrlich ascites tumor cells with alkylating agents leads to the formation of DNA-protein complexes resistant to phenol-salt deproteinization procedures. De pending on the salt used, this effect results either in decreased amounts of DNA extractable into the aqueous phase of the extraction mixture or in increased amounts of protein bound to the DNA obtained in the aqueous phase. If ...
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Inhibition of fermentation or of glucose uptake in the presence ofoxygen has been observed in many forms of life and is generally referred to as the 'Pasteur effect'. In most tissues, oxidative inhibition of fermentation (ethanol or lactate production) is reflected by a parallel decrease in glucose consumption. Since certain tissues utilize lactate significantly, estimation of the Pasteur effec...
متن کاملIncreased histone acetylation and deacetylation in rat ascites hepatoma cells.
Rates of histone acetylation and deacetylation in nuclei from fetal, adult, and two kinds of neoplastic rat hepatocytes were examined. Histone acetylation in isolated nuclei was measured in the presence of 6 mM sodium n-butyrate, a potent inhibitor of deacetylase, and in the absence of the inhibitor. The deacetylase activity was estimated from the difference between the rates with or without th...
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ژورنال
عنوان ژورنال: Chemical and Pharmaceutical Bulletin
سال: 1977
ISSN: 0009-2363,1347-5223
DOI: 10.1248/cpb.25.812